Freshwater ecosystems are particularly vulnerable to direct and indirect anthropogenic stressors, leading to shifts in fish communities in response to environmental changes. Traits that mediate fish community responses to altered environments by influencing species' survival and adaptability can inform individual species and fish communities responses to changing environments. Here, we use a trait-based approach to predict how stream fish communities vary in response to environmental and land use change in five U.S. ecoregions: the Columbia, Upper Colorado River, and Mobile Basins, and the Great Lakes and Chesapeake Bay watersheds. We examine changes in habitat suitability for native, non-native, and game fishes from changing environments. By integrating trait-based models with landscape-scale data, we can refine predictions of ecosystem responses and better inform sustainable resource management.