The widespread use of antibiotics in animal agriculture has increased the environmental prevalence of antimicrobial resistance genes (ARGs) which poses a growing public health concern. Streams can facilitate the downstream dispersal of ARGs while benthic biofilms can remove ARGs from the water column, influencing persistence and propagation. Despite a growing body of literature on environmental ARGs, the relative influence of water temperature and benthic biofilms on the removal of ARGs from the water column remains understudied. We predict that stream warming will increase biofilm biomass thereby altering the fate of ARGs through water column removal. Using n=24 temperature-controlled recirculating mesocosms at the Notre Dame Experimental Mesocosm Facility (ND-EMF), we tested the interacting effects of water temperature (20° C vs. 26° C), biofilm presence (colonized vs. bare), and the presence of autotrophic vs. heterotrophic constituents using inorganic (rock) and organic (cellulose) substrata on ARG removal rates. We conducted pulse additions of a ARG-rich manure slurry, and collected sequential water samples from each mesocosm at five time points (0.5, 2, 4, 10, and 24hrs) to quantify ARG removal rates (as k; hr-1). For each filtered water sample, using digital PCR, we measured concentrations of ARGs for tetracycline (tetW) and erythromycin resistance (ermB), as well as a ruminant fecal indicator (bacR), and a bacterial marker gene (16s rRNA). We hypothesized that warmer temperatures would increase removal rates through augmented biofilm biomass, which would promote the physical removal of ARGs. Additionally, we hypothesized that the algal growth on the organic substrata would not degrade ARG-containing particles, while heterotrophic biofilm communities colonized on the inorganic substrata would. Previous research has demonstrated that warmer water temperatures can increase the water column removal of other molecular constituents (e.g., environmental DNA), but warming has not been examined specifically for ARGs. While the molecular detection results of this experiment are forthcoming, determining how water temperature influences ARG transport in streams will help mitigate the spread of antibiotic resistance in aquatic ecosystems.