Aquatic invertebrate community composition is a standard measure of freshwater ecosystem health. Monitoring of invertebrate communities usually focuses on taxa such as Trichoptera, Plecoptera, or Ephemeroptera. Collectively referred to as EPTs, these taxa are widely studied owing to their nutrient cycling capabilities and water quality sensitivity (Lenat, 1988). However, their unreliable presence in some unstable and remote environments such as ephemeral alpine pools, creates a challenge in assessing a full range of ecological conditions. Chironomids represent a widespread and abundant taxon that remain understudied due to the technical challenges of their identification, especially in remote areas where invertebrate sampling rarely occurs (Ward et al. 2002). This research aims to investigate how Chironomidae subfamily diversity and abundance reflects biotic and abiotic conditions of ephemeral aquatic habitats in the alpine Green Lakes Valley. These bodies of water represent a model system due to reduced direct anthropogenic influence. We aim to quantify and categorize the benthic invertebrate taxa using invertebrate and environmental samples collected from 2021 to 2024 and compared with historical data collected in 1987-88 (Mihuc and Toetz, 1996). All specimens were identified using light microscopy, and chironomids were slide-mounted and identified to subfamily and genus when possible. The changes in chironomidae subfamily composition may reflect the availability of genera-specific trophic conditions due to the presence or absence of prey. Our findings represent a step towards developing Chironomids as an indicator taxa for alpine aquatic research in the face of volatile future climates.