More than 76,000 stormwater ponds (SWPs) have been constructed in Florida to control organic and inorganic nutrient loadings from the surrounding landscape. Human decisions on SWP design and management control external nutrient inputs, potentially altering the form and magnitude of nutrient availability and subsequently influencing microbial metabolism within SWPs and downstream habitats. Microbes can excrete extracellular enzymes to acquire metabolic organic substances when there is a relative limitation of bioavailable carbon (C) or inorganic nitrogen (N) and phosphorus (P) for their metabolism. Therefore, extracellular enzyme activities (EEA) can reflect the microbial demand for, and environmental availability of, organic nutrient subsidies. However, the quantity and quality of dissolved organic matter (DOM) that control microbial nutrient (C, N and P) demand that may predict nutrient removal by SWPs are poorly understood. Furthermore, seasonal shifts in DOM composition and inorganic nutrient availability, driven by the imbalance between terrestrial inputs and autochthonous production, may alter microbial nutrient acquisition strategies, although this remains unclear. We sampled 19 SWPs from two master-planned communities (GNV and LWR) throughout 2025-2026 to understand how microbial nutrient acquisition strategies responded to the amount, sources, chemical composition and bioavailability of DOM and inorganic nutrient availability across wet and dry seasons. We incorporated DOM characterization and extracellular enzyme assays to better understand microbial reliance on organic substrates. We hypothesized that, spatially, SWPs with algal blooms will have a larger fraction of bioreactive DOM but an inorganic nutrient deficit, leading to high N-, P- and labile C-acquiring enzyme activities. Seasonally, we expect that N-, P- and labile C-acquiring enzyme activities will be positively correlated with N- and P-containing DOM compounds during the summer wet season. Preliminary results revealed strong co-limitation (C and N) for microbial uptake in GNV, whereas P and C were co-limiting in LWR, suggesting that different pond management can alter microbial metabolism. In the hot, wet season, the N- and P-acquiring enzyme activities were constrained by the inorganic nutrient deficiency and terrestrial organic carbon inputs. This study will improve our understanding of microbial transformations of organic carbon, potential nutrient limitation and microbial function from human-made aquatic ecosystems.