The hyporheic zones of rivers or estuaries are key areas for biogeochemical reactions due to the high surface area for microbial populations and a constant influx of reactants. Microplastics have been observed extensively in aquatic environments and have been shown to alter biogeochemical processing and stimulate greenhouse gas production because they increase surface area for microorganisms and become a source of dissolved organic carbon as they degrade. The size, shape, concentration, and type of plastic have been shown to influence microbial colonization and the rate of degradation. In this pilot study, we used column experiments to simulate hyporheic flow through sediments with different types of microplastics, including three fossil-fuel derived plastics: polypropylene (PP), polyethylene (PE), polyethylene terephthalate (PET), and one plant-derived, biodegradable plastic: polylactide (PLA). Microplastic particles of uniform initial size, shape, and concentration were distributed through identical 1-m long columns, through which water was continuously pumped for 10 weeks. During flow, dissolved oxygen, nitrate, nitrite, and ammonia concentrations were measured in pore waters sampled every 10 cm along the flow path in each column. At the conclusion of the experiment, DNA extracted from the sediments at the sampling locations was analyzed to determine microbial abundances. We observed the development of aerobic and anaerobic zones along each flow path and the influence of each type of plastic, and this pilot project will be the basis for planning future studies of the influence of additional microplastic properties, including size, shape, and concentration.