In urban watersheds, small order streams face a variety of pollution delivered through surface and subsurface runoff. But the composition of the runoff changes depending on the season; higher instances of nutrient pollution in the summer and increased salt pollution in the winter. Pollution often affects the function and metabolism of a stream by influencing respiration of microbial communities. We look to explore the seasonal variations in the production of greenhouse gases (GHG), including carbon dioxide, methane, and nitrous oxide, as a measure of the system’s response to pollution. The study site is Flat Branch Creek in Columbia, Missouri. Flat Branch Creek’s watershed is 8.31 square kilometers with 97% of the watershed possessing slow or very slow infiltration rates. Flat Branch Creek experiences high levels of pollution due to heavily developed land use coupled with poor infiltration and high runoff. Flat Branch Creek is currently a sample site for a larger synoptic sampling project. This data has demonstrated that the stream experiences variations in chloride levels of 31 mg/L to 690 mg/L, total dissolved nitrogen levels of 0.13 mg/L to 1.37 mg/L, and total dissolved phosphorus levels of 0.012 mg/L to 0.15 mg/L. Furthermore, during present sampling, conductivity levels have averaged around 1000 μS/cm but experienced a 5-fold increase to 5000 μS/cm after the first snowfall and melt. The high variability in stream chemistry demonstrates the need to understand the response to dynamic pollution within the stream, hyporheic zone, and riparian zone. Across a transect of the stream riparian interface, we placed wells and soil collars for measurement at stream surface, bank, and riparian zone. From October to March, weekly sampling events occur to collect surface and hyporheic water and GHG flux. YSI probes are used to measure the dissolved oxygen, oxidation-reduction potential, and conductivity. In the lab, filtered samples are analyzed for total dissolved nitrogen, total dissolved phosphorus, nitrate, ammonium, and dissolved organic carbon. Through exploring the biogeochemistry of the stream-riparian interface over the course of three seasons, we expect to see the influence of urban pollution on the stream-riparian interface’s GHG production.