Fine organic particles are an important food resource in streams. They also serve as a key vector linking the benthos to the water column and upstream reaches to downstream ecosystems. However, few studies have assessed whether benthic and suspended fine particles are a single pool or if suspended particles are qualitatively different from those on the bed. This study examined temporal and spatial patterns in the composition of seston and benthic fine particles across 20 streams in the South Sandy Creek Watershed, Alabama. The watershed is approximately 95% forested and is dominated by a mixed canopy of Longleaf (Pinus palustris) and Loblolly (Pinus taeda) Pines, with an understory of Oak and other hardwoods. We collected seston and benthic fine particles monthly and characterized particle abundance, size distribution, and an array of shape and color characteristics using FlowCam 8000 with fluorometric detection. Benthic organic matter was quantitatively collected using a stand-pipe sampler, and partitioned into fine (10-100 µm) and coarse (>100 µm) fractions. Ash-free dry mass, percent organic matter, and isotopic signatures were also estimated for both seston and benthic fine particles. Preliminary results from September to December 2025 indicate that seston concentrations were highly variable and lacked a clear longitudinal pattern (0.1 km² to 123 km²). Averaged across sites, September had the highest concentration of particles in suspension (3,014 particles/ml) and concentrations were lowest in November (1,578 particles/ml). The three sampling locations with the largest discharge had the most stable concentrations over time (ranging between 2,000 and 5,000 particles/ml), whereas headwater sites showed extreme variability (~300 - > 20,000 particles/ml). We are currently analyzing benthic and suspended particles for elemental content and isotope signatures to better characterize each pool. Preliminary results suggest that fine particle dynamics (e.g. suspension, transport, and deposition) are much more episodic and variable in headwater streams than in downstream reaches that integrate runoff from larger geographic areas. When completed, this research will improve our understanding of the coupling between benthic and suspended fine particles in streams and how they contribute to downstream export of energy and elements in lotic ecosystems.