Intermittent rivers make up at least 30% of the total length and discharge in river networks throughout the globe. Intermittent rivers are estimated to be more abundant in the future due to the effects of climate change and water abstraction. Data from previous work indicate that intermittent streams have lower bacterial/archaeal diversity and greater abundance of intermediate-lability and complex organic matter molecules. This project aimed to expand previous work and understand the hydrological conditions at which stream bacterial communities destabilize and shift as stream water dries after a rainfall event in intermittent stream reaches in an urban watershed in a large, metropolitan area: San Antonio, TX. After a ~2-inch rainfall event on June 12, 2025, three intermittent and three perennial stream reaches were sampled for water chemistry, water and sediment bacterial/archaeal communities (via 16S rRNA gene amplicon sequencing) between mid-June and mid-August during dry-down after flow returned. Preliminary results indicate that nitrate concentrations are higher in perennial streams relative to intermittent. Kruskal-Wallis test results indicate that differences in nitrate concentration are significant (p = 0.03). Orthophosphate showed similar patterns to nitrate. However, the differences in orthophosphate concentrations in both stream types were deemed marginally significant based on the Kruskal-Wallis test (p = 0.051). This is likely due to perennial rivers in this region being supplied with nutrient-rich water from wastewater treatment plants and other industrial runoff. Future work will include measurement of the dehydrogenase enzyme (proxy for aerobic respiration) and characterization of the water and sediment microbiomes.