Aquatic macroinvertebrate data have long been used as core indicators of ecosystem health and productivity. The National Ecological Observatory Network (NEON) is a research platform designed to assess the effects of climate, land-use change, and invasive species across North America. NEON’s aquatic program consists of 24 streams, 7 lakes, and 3 rivers across 19 eco-climatic domains. NEON’s macroinvertebrate data collection began in 2014 with samples collected in the two most common habitats at each site. Samples are collected three times per year, roughly in spring, summer, and fall, then identified by expert taxonomists to the lowest practical taxonomic level and measured to the nearest mm. These data enable analyses of diversity and community metrics, organismal abundance, and biomass estimates over space and time. Within NEON macroinvertebrate data, alpha diversity is highest in streams with seasonal snowmelt pulses like Blacktail Deer Creek (BLDE), MT (50.6) and Teakettle Creek (TECR), CA (46.8), and lowest in streams with seasonal drying like Kings Creek (KING), KS (18.7) and Sycamore Creek (SYCA), AZ (19.3). Macroinvertebrate density is also highest in snowmelt-dominated streams such as West St. Louis Creek (WLOU), CO (44,530 organisms m-2) and TECR (34250 organisms m-2), however, abundance at the sites with seasonal drying falls in the middle of the range (KING = 11037 and SYCA = 21817 organisms m-2), with low macroinvertebrate abundance occurring in the northern latitude streams Oksrukuyik Creek (OKSR) and Caribou (CARI) Creek, AK (2421 and 1735 organisms m-2, respectively). Patterns in primary producers will also be explored through this analysis. These data leverage multiple NEON data products, including Macroinvertebrate Collection (DP1.20120.001), Periphyton, seston, and phytoplankton chemical properties (DP1.20163.001), and Continuous Discharge (DP4.00130.001) to explore patterns in the stream macroinvertebrate community across space and time.