Poster Presentation Society for Freshwater Science 2026 Annual Meeting

Concentration-Discharge Hystereses Reveal the Dominant Hydrological and Biological Controls on Nitrate Concentration Dynamics along the Upper Clark Fork River, Montana, USA (135714)

Rylie Dupuis 1 , Robert A. Payn 1 , Micheal D. DeGrandpre 2 , Marc Peipoch 3 , Juliana D'Andrilli 4
  1. Montana State University, Bozeman, MT
  2. University of Montana, Missoula, MT
  3. Stroud Water Research Center, Avondale, PA
  4. University of North Texas, Denton, TX

The Upper Clark Fork River (UCFR) in western Montana is part of the largest Superfund project in the United States and has undergone extensive restoration following decades of mining-related contamination. Monitoring associated with ongoing floodplain restoration activities, including contaminated sediment removal and floodplain reconnection, provides a unique opportunity to examine long-term nitrate dynamics in a heavily managed river system. This study examines how the coupling of seasonal ecosystem uptake with a snowmelt-driven hydrologic regime influences temporal and longitudinal patterns of nitrate concentrations in the UCFR. We analyzed nitrate as NO₃–N from 13 sites spanning ~200 km of the UCFR, sampled biweekly to monthly in a growing dataset from 2017 to 2024. We hypothesized that the primary controls on seasonal nitrate patterns are flushing or dilution during the snowmelt season and uptake of nutrients by algae during the growing season, and that spatial differences in nitrate loading from groundwater produce distinct concentration-discharge (C–Q) relationships along the river. We present consistent evidence from the hystereses of C–Q curves at most monitoring sites that nitrate concentrations rapidly decrease, likely due to dilution at peak flows; remain low during decreasing and warm low-flow conditions during the algal growing season with increased biological demand; and increase during cooling low flows due to higher concentrations in groundwater inputs and decreasing ecosystem uptake. The spread of these hystereses appears to increase in upstream segments of the river, where groundwater nitrate loading is stronger, and then decrease downstream where groundwater nitrate loading is weaker. Comparisons of two UCFR tributaries representing contrasting land-use influences provide some evidence that sites with very low nitrate loading from groundwater may exhibit little to no hysteresis and either chemostasis or a modest flushing effect of higher nitrate concentrations at high flows. Together, these results demonstrate how hydrology, nitrate loading, and biological uptake interact to control nitrate dynamics in a recovering river system with a snowmelt-dominated hydrologic regime. 

  1. Beegum, S., Malakar, A., Ray, C., & Snow, D. D. (2023). Importance of snowmelt on soil nitrate leaching to groundwater: A model study. Journal of Contaminant Hydrology, 255, Article 104163. https://doi.org/10.1016/j.jconhyd.2023.104163.
  2. Fazekas, H. M., Royer, T. V., & Bernhardt, E. S. (2020). Dissolved organic carbon and nitrate concentration–discharge behavior across scales: Land use, excursions, and misclassification. Water Resources Research, 56.​
  3. Ohte, N., Sebestyen, S. D., & Kendall, C. (2004). Tracing sources of nitrate in snowmelt runoff using a high-resolution isotopic technique. Journal of Geophysical Research, 109(G4).​
  4. Pellerin, B. A., Saraceno, J., Shanley, J. B., Sebestyen, S. D., Aiken, G. R., Wollheim, W. M., & Bishop, K. (2011). Taking the pulse of snowmelt: In situ sensors reveal seasonal, event, and diurnal patterns of nitrate and dissolved organic matter variability in an upland forest stream. Journal of Geophysical Research, 116(G3).​
  5. Piatek, K. B., Mitchell, M. J., Silva, S. R., Kendall, C., & McHale, P. J. (2005). Sources of nitrate in snowmelt discharge: Evidence from water chemistry and stable isotopes. Water, Air, & Soil Pollution, 165, 13–35. https://doi.org/10.1007/s11270-005-4641-8.​
  6. Valett, H. M., D’Andrilli, J., DeGrandpre, M. D., Payn, R. A., & Peipoch, M. (2017). LTREB: Collaborative research – River ecosystem responses to floodplain restoration (NSF Grant No. DEB-1655197). University of Montana.​
  7. Valett, H. M., Peipoch, M., & Poole, G. C. (2022). Nutrient processing domains: Spatial and temporal patterns of material retention in running waters. Freshwater Science, 41(2), 195–214. https://doi.org/10.1086/719991.​