Intermittent streams, which comprise a large proportion of lotic systems globally, exhibit unique hydrological dynamics that may influence the transport and ecological effects of insecticides. During low-flow periods, isolated pools can accumulate solutes, which are released downstream as high-concentration pulses once flow resumes. We investigated the distribution and ecological effects of imidacloprid, a neonicotinoid insecticide widely used to treat Eastern Hemlock (Tsuga canadensis) for Hemlock Woolly Adelgid (Adelges tsugae), and its metabolites (imidacloprid-olefin, desnitro-imidacloprid, and imidacloprid-urea) in intermittent stream systems. While imidacloprid is highly toxic to aquatic macroinvertebrates, prior research has found limited impacts on downstream communities following applications near perennial stream systems, leaving the effects in intermittent systems poorly understood. We investigated these trends in forestry lots in Western New York state using a three-fold approach. First, we tracked continuous hydrological conditions in three stream systems, including two treatment sites and one untreated control, using Stream Temperature, Intermittency, and Conductivity (STIC) sensors. Second, we quantified concentrations of imidacloprid and its metabolites in surface water and sediment using liquid chromatography tandem mass spectrometry (LC-MS/MS), comparing among sites differing in flow regime (intermittent vs. perennial) and location relative to treatment sites (upstream vs. downstream). Finally, we assessed the composition and biodiversity of the aquatic macroinvertebrate community to evaluate the combined effects of insecticide concentration and flow intermittency on non-target aquatic invertebrates. Preliminary results suggest imidacloprid application has little impact on invertebrate community composition in intermittent streams. This may be related to relatively low concentrations in winter surface water samples, though spring water samples and sediment samples are still in progress. This study will provide insight into the sustainability of current integrated pest management practices for Hemlock Woolly Adelgid by evaluating whether existing stream management zone definitions effectively protect aquatic habitats and their non-target macroinvertebrate communities from insecticide accumulation and transport.