Headwater streams play an essential role in biogeochemical cycling via the carbon metabolism and nitrogen transformations mediated by benthic biofilms. As climate change progresses, higher temperatures will co-occur with more frequent and intense drought disturbances, with unknown consequences for stream ecosystem function. We tested the effect of warming on stream biofilm recovery from drought in an outdoor mesocosm experiment by measuring two key biofilm processes: metabolism and dinitrogen (N2) flux. We inoculated the flumes with biofilms from nearby streams and maintained ambient and warmed (+5°C) treatments for one year before subjecting all flumes to a 1-month drought. We measured biofilm metabolism before and after drought by incubating biofilm-covered stones in recirculating chambers and estimated rates gross primary production (GPP) and community respiration (CR) as the change in dissolved oxygen over time in light and dark conditions, respectively. To measure potential N2 flux, we collected dissolved N2 gas samples at the start and end of the incubations. We measured biofilm chlorophyll a concentration and ash-free dry mass from each set of stones. Our data show increased GPP (g O2/g Chl-a/hr) in the warmed flumes after drought, and a decrease in ambient flumes. Additionally, CR (g O2/g AFDM/hr) was similar between temperature treatments and was lower following drought. Preliminary N2 flux results show evidence of N2 fixation by both ambient and warmed biofilms. Our data provide evidence that long-term warming may affect the recovery of benthic biofilm metabolism after drought, suggesting that metabolic responses to disturbance are influenced by temperature regime.