Urbanization impacts the invertebrate communities in aquatic ecosystems, typically causing species assemblages to be dominated by pollution-tolerant, generalist taxa. Less is known, however, about how urbanization alters intraspecific traits in aquatic organisms. Intraspecific variation in behavioral traits is particularly important because the behavior of aquatic organisms can have significant impacts on ecosystem functioning and trophic relationships. We hypothesized that aquatic invertebrates from urban environments would be more bold, active, and voracious foragers relative to conspecifics from less-impacted systems, since predation pressure, a major selective force on behavioral traits, tends to decrease with increasing anthropogenic impact. To test this hypothesis, we collected peninsula crayfish (Procambarus paeninsulanus) from streams with either predominantly developed or forested watershed land use in and around Gainesville, Florida. We put crayfish through three separate laboratory assays to test their latency to emerge into a novel environment (i.e., boldness), foraging voracity, and activity levels. Our preliminary findings suggest crayfish from forested streams may have spent a greater proportion of time active, a lower proportion of time utilizing refuges, and foraged more rapidly than crayfish from urban streams, all of which contradict our hypothesis. Crayfish from both types of streams displayed similar boldness. We plan to expand upon this initial work by collecting and assaying crayfish from urban and forested ponds, and to determine the generality of our findings by conducting a similar experiment on another aquatic invertebrate species in a different order (Odonata). Changes in crayfish behavior that result from urbanization could be ecologically important, as crayfish often represent the dominant benthic consumers in aquatic habitats, and their behavior can have appreciable impacts on the functioning of these ecosystems.