This assesses the correlation between seasonality in macroinvertebrate assemblage structure and its implications for rapid bioassessment results. Changes have been observed in the composition of the macroinvertebrate assemblage across seasons at a long-term monitoring site in North Alabama, which inspired this project. This has led to varying results of stream conditions (e.g., excellent vs. good. vs. fair) across seasons at that site. Due to funding restraints, most biomonitoring programs can only afford to take one sample per site per year, which could lead to improper classification of stream quality. By understanding the effects of seasonality on macroinvertebrate bioassessments in our region, biologists would have more information to choose the best time of year to conduct sampling efforts. This study will be conducted in the Uchee Creek Watershed, a tributary to the Chattahoochee River and a Strategic Habitat Unit for Alabama which contains many rare, threatened, and endangered aquatic species. We collected samples during the summer, fall, and winter seasons from 10 sites in first and second order streams downstream of road-stream crossings. Twenty sampling efforts were completed using a D-Net to survey all available habitat types over a 100 meter reach. Collected macroinvertebrates were preserved in 70% ethanol and identified to Order in the laboratory. The taxon richness, percentage of the Dominant Taxon, Bioassessment Score, and other metrics were calculated for each sample. We used ordinations such as nonmetric multidimensional scaling to examine the differences between macroinvertebrate assemblages across the seasons.