Total suspended solids (TSS) in streams are influenced by both environmental and anthropogenic drivers. Freshwater biota and watershed health are shaped by both volatile suspended solids (VSS) and nonvolatile suspended solids (NVSS) in streams. Volatile solids represent the organic material in streams that are key for aquatic health. Nonvolatile solids represent the inorganic stream solids (silts/clays) that influence water clarity, sedimentation, and abrasion. This study sets out to investigate how VSS and NVSS vary with land-use cover and seasonality over the course of one water year. Thirteen stream sites in Southwestern Ohio with differing land-use percentages (e.g. urban vs. agricultural), including two directly impacted by wastewater treatment plant discharge, were selected for this study. Biweekly water samples were collected and analyzed for TSS, VSS, NVSS, and VSS% using filtration and loss on ignition methodology. Complementary analysis of carbon-nitrogen (C:N) results were used to quantify the particulate organic carbon (POC) of each sample. We anticipate higher VSS% within agricultural watersheds due to increased canopy cover in riparian areas and higher stream nutrient concentrations leading to increased in-stream production. During cold and wet periods, we anticipate increased NVSS% due to increased runoff and decreased in-stream production. We expect low C:N ratios in agricultural watersheds due to accelerated decomposition and nutrient cycles, and low C:N ratios in urban streams with increased storm-water runoff and reduced vegetation in riparian zones. POC measured by C:N is anticipated to show seasonal variation based on shifts in organic content and in hydrologic changes over the water year. This integrated approach will advance understanding of how watershed land-use and seasonal trends shape the organic and inorganic suspended solids in streams for both ecological assessments and watershed management.