Microplastic pollution is an understudied issue that impacts freshwater ecosystems and the Great Lakes ecosystems in particular. This study investigates the abundance and type of microplastics in the Tittabawassee-Saginaw River in Michigan, which drains to the Saginaw Bay of Lake Huron, to assess the impact of the upstream-downstream gradient, wastewater treatment plants (WWTPs), and local land use. Water samples were collected from 12 sites and the particles were identified using a dissecting microscope. The main microplastics shape were clear fibers and no other shapes were found in enough abundance for analysis. Statistical tests revealed no significant relationship between microplastics and the upstream-downstream gradient (p=0.24) and there was no significant difference found between samples upstream and downstream of WWTPs (p=0.88). Among the land use types only “developed open-space” showed a significant positive correlation with microplastic concentration (p=0.01).