North American beaver (Castor canadensis) are ecosystem engineers known to alter physical and chemical processes through hydrologic manipulation, and influence biogeochemical processes and downstream water quality. However, the spatial patterning of biogeochemical processes and nutrient storage within beaver-created ecosystems is not well understood. We aimed to investigate spatial variation of denitrification and soil carbon and nutrient (nitrogen, phosphorus) stocks in a beaver-created ecosystem in northeastern Connecticut, USA. We collected soil cores to a 30 cm depth, divided into 10 cm increments, at random points (n = 20) throughout three vegetation zones (open water, emergent wetland, and meadow) in August 2025. Denitrification potential was estimated using denitrification enzyme assays. Preliminary analysis suggest denitrification potential was highly variable throughout the beaver-created ecosystem, ranging from approximately zero to > 3900 ng N hr-1 g soil-1. There did not appear to be a clear spatial pattern relating to dam proximity or vegetation zone. However, the highest points appeared to be located adjacent to shrubs and woody vegetation, including alder, a nitrogen-fixing species. Throughout the system, denitrification potential appeared highest and most variable in the top 10 cm of the core, where microbes are most active. Log-transformed denitrification potential was positively correlated to soil moisture in the top 20 cm and negatively correlated to bulk density in the top 10 cm. Soil carbon and nutrient stock processing is ongoing. Our results may allow for a more accurate understanding of how beavers influence nutrient storage and processing behind dams and inform management decisions regarding increased beaver populations in the northeastern United States.