Microplastics are a contaminant of emerging concern, but very little is known about their presence and movement in freshwater ecosystems, and how seasonal changes can affect that movement, especially in the Appalachian Mountains. With the Appalachian region being the headwaters of many rivers, this experiment was conducted to begin filling the gaps in our understanding of microplastics in freshwater across the changes in seasons. By examining if there is a significant amount of microplastics being added to the Davidson River, which has its headwaters in the Pisgah National Forest, two sample sites were selected based on proximity to the headwaters and the mouth of the river. Three water samples were taken from each location, as well as a control blank sample, each size fractioned at 125 µm, 250 µm, and 500 µm. The samples were then separated first by density with CaCl₂, then by digestion of organic materials, before being filtered to be counted under a microscope. Seasonal changes affect water flow, which could impact how microplastics disperse and the overall impact they have on freshwater ecosystems. The findings of this study will provide insights into the effects of changes in climate on the distribution and dynamics microplastics pollution in river ecosystems.