Human modification of freshwater flows is widely associated with shifts in carbon pools and fluxes, most commonly studied in urban and developed landscapes. However, flow modification also occurs in otherwise pristine ecosystems for unique human-wildlife management purposes. Kruger National Park (South Africa) is a 19,000 km2 savanna landscape that contains more than 40 dammed impoundments constructed along intermittent streams to provide dry-season water resources for wildlife. These impoundments aggregate large herbivores (e.g., hippopotami, elephants, giraffes), which can further influence aquatic carbon biogeochemistry through physical disturbance, grazing, and subsidies of organic matter and nutrients. We investigated how artificial impoundments and variation in large herbivore density and diversity influence carbon cycling in a savanna grassland landscape by comparing dammed impoundments to unaltered intermittent streams. Across 11 watersheds, we selected paired sites consisting of one intermittent stream and one dammed stream (hereafter, impoundment). Impoundments feature a gradient of low to high large herbivore density. Impoundments spanned a gradient of low to high large herbivore density. At each site, we sampled three positions along an elevation gradient spanning the terrestrial-aquatic interface: upland (rarely inundated), transitional, and bank (seasonally or persistently inundated). At each position, we measured carbon dioxide and methane fluxes and collected 30-cm deep soil and sediment cores to quantify carbon stocks and bacterial abundance. Preliminary dry season gas flux comparisons between ephemeral stream and impoundment transects show that uplands are similar in both carbon dioxide and methane fluxes, and that ecosystem types become increasingly divergent in transitional and bank zones. Specifically, carbon dioxide is greater, and methane is lower in impoundments compared to intermittent streams in transitional and bank elevations. We further expect that impoundment carbon stocks and bacterial abundance will increase from upland to bank zones, and that transitional and bank zones in impoundments will be greater than those of intermittent streams due to longer hydroperiods. This study provides a unique opportunity to study how human modification of surfaces waters influences biogeochemical pools and fluxes in undisturbed savanna landscapes, and how large herbivorous wildlife mediate these functional properties.