Governmental organizations, nonprofits, universities, neighborhood groups, and independent individuals engage in stewardship behaviors to protect and care for the Little River Watershed in Miami, Florida. The large number of stewards makes it challenging to keep track of everyone’s stewardship efforts. As a result, organizations may engage in redundant or conflicting activities, miss out on relevant collaborations, or otherwise engage in stewardship that could be more effective if better organized. To support stewards in performing stewardship as efficiently and effectively as possible, we surveyed and interviewed organizations and individuals that care for the Little River Watershed. We used thematic coding, spatial analysis, and social network analysis to identify trends in a) stewardship locations, b) activities, and c) patterns of collaboration. We found that stewardship of the watershed is focused on the eastern portion of the river in public access parks. Stewardship functions ranged from community engagement (> 75% of surveyed stewards) to monitoring (50% of surveyed stewards). Organizations were not always connected with groups with similar activities, stewardship locations, or mission statements. Ocean conservation groups were the most aligned in stewardship locations and activities. Capacity in terms of time and resources emerged as a key theme in our analysis of stewardship barriers. These findings highlight areas of improvement for Little River stewardship including connecting potential collaborators, initiating stewardship in the western reaches of the river, and identifying resources for capacity building. Overall, this research strategy generated useful insights and action areas for supporting efficient care of the Little River and collaboration of diverse organizations. Researchers and managers should consider this stewardship mapping and assessment method to organize and better understand the watersheds in which they work.