Non-native, invasive species represent one of the greatest threats to freshwater biodiversity. For example, the freshwater jellyfish Craspedacusta sowerbii is native to the Yangtze River valley in China but has established populations in multiple Texas reservoirs, where its presence may disrupt food webs through voracious zooplankton grazing. In Texas, this species was first detected at Medina Lake near San Antonio and has since spread to other reservoirs near Dallas to the north and Houston to the east. The jellyfish spreads through connected river systems and human activity such as recreational boating. However, limited understanding exists about the historical spread and establishment of this jellyfish, and their distribution remains understudied. Therefore, we conducted an environmental DNA (eDNA) survey to assess the spatial distribution of C. sowerbii in reservoirs of central Texas. Specifically, we collected surface water at publicly accessible boat ramps and conducted on-site filtration before returning to the lab for quantification of jellyfish eDNA via qPCR. Moreover, we took advantage of the fact that our research team had previously collected samples at our study sites (years = 2014, 2020), repurposing old samples to examine invasion chronology through time. Occurrence data from our study will support state and local agencies in developing more effective management strategies to protect Texas freshwater ecosystems from invasive species and enhance conservation by identifying invasion hotspots.