The Edwards (Balcones Fault Zone) Aquifer is a highly productive karst aquifer system in the south-central region of Texas that serves as the primary water source for over 2.5 million individuals. Viability of this natural aquifer system is largely reliant on basal stream flow over the contributing zone, and lateral subsurface flow from the adjacent Trinity Aquifer. Surface water originating from the Edwards Plateau catchment, which overlies the Trinity Aquifer and is considered the contributing zone of the Edwards (BFZ) Aquifer, directly recharges the Edwards (BFZ) Aquifer by infiltrating Edwards limestone outcrops in the Balcones Fault Zone. Resident microbiota of groundwater are evolved to survive unique nutrient and physical conditions of the subsurface environment and are primary drivers of biogeochemical cycling within groundwater. Consequently, recharge events have the capacity to alter typical microbial signatures as well as dominant metabolic processes upon the introduction of water with differing chemistry. Temporal sampling of groundwater from the contributing and recharge zones of the Edwards (BFZ) Aquifer occurred quarterly from March 2024 to December 2025. Two additional sampling events occurred to include contributing surface river water and a significant recharge event in May and June 2025, respectively. Approximately 60L of groundwater were collected at wells at each sampling events, except riverine water had only 3L collected, and microbial cells were collected via vacuum filtration through 0.45 µm filters. Targeted 16S rRNA gene amplicon sequencing and shotgun metagenomic sequencing were utilized to identify the taxonomic structure of bacterial and archaeal communities as well as their metabolic potential. Major ions and other important geochemical parameters were also collected at the time of sampling. Geochemistry analysis demonstrates spatially variable, and temporally stable groundwater chemistry within the contributing and recharge zones of both Trinity and Edwards aquifers. PERMANOVA testing of Bray-Curtis dissimilarity metrics indicates that aquifer and sample site significantly impact community composition, with 14.7% and 34.4% of the variation between samples explained by aquifer and site, respectively. Further analysis of the metagenomics dataset in conjunction with geochemistry and 16S data will give insight to the metabolic functionality of the microbial consortia present across the Edwards (BFZ) Aquifer.